RNA extraction a) Coupling of antigen to the column b) Ammonium sulphate precipitation of IgG http://www.ncbi.nlm.nih.gov/pmc/articles/PMC380730/pdf/applmicro00053-0040.pdf c) Antibody purification Immuno-fluorescence (IF): Immuno-precipitation (IP) Affinity purification of proteins a) GSH purification b) Ni-NTA purification c) GBP protocol: |
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