We are a team of individuals who are interested in understanding reaction mechanisms in Biological systems and manipulating the activity of such systems by protein engineering for purposes relevant to environment and industry.
We are looking for motivated enthusiasts for the team who are willing to take challenges at the interface of chemistry and biology. A background in Biochemistry is desirable but not essential. Please contact us with a copy of your CV and motivation letter if you are interested.
Saeed defends his MS thesis.
Welcome Akshaya and Indhu! Akshaya will carry out her iPhD project and Indhu will work on her BS/MS final year project.
Nature utilizes oxidoreductases to cater for its energy needs in respiratory processes.Oxidoreductases are enzymes that catalyze redox reactions relevant to the normal functioning of cell. The active sites of oxidoreductases usually contain transition metal ions and organic cofactors. Amino acid residues in the primary and secondary coordination sphere modulate the redox potential of the metal ions to carry out specific reactions. A recurring theme of the research on redox enzymes is to understand electron/proton transfer processes. Substantial efforts have been made to understand how the insulating protein matrix is capable of conducting electrons/redox equivalents on the ms–μs timescale within (and in between) these enzymes and proteins. The sophisticated experimentation combined with the development of electron-transfer theory has taught us a lot about certain enzyme systems. However, it is important to admit that we are still not able to engineer biomimetic systems which can perform similar tasks in a predictable manner.
The Biocatalysis and Bioenergentics Laboratory team at IISER Bhopal focuses on applying novel single-molecule and ensemble techniques to study electron-transfer and catalysis during enzyme turnover which is complementary to the customary single-turnover or pre-steady-state kinetics methods to yield a complete picture about catalysis. Current efforts of the team are in engineering proteins for the purpose of harnessing solar energy to drive biocatalytic reactions of interest to the industry and environment. Specific details about the projects are mentioned below.
Currently there is growing need of renewable or alternative sources of energy as the sources of fossil fuels are depleting rapidly. Solar energy is available in abundance and if utilized appropriately holds tremendous potential to solve the issue of energy crisis. Recent progress in photovoltaics is promising but the technology is limited by poor quantum yields. Our approach in this direction is to engineer proteins for light harvesting and storing the solar energy in the chemical bond - the primary source of energy. We do so by attaching photoredox catalysts in the vicinity of enzyme active site so that there is no transfer of electron between the photocatalyst and enzyme active site in the ground state but upon excitation with visible light, electrons can flow from the photocatalyst to the enzyme active site which can utilize the same for its normal function.
Photoredox catalysis has sought much attention in the past decade due to its inherent selectivity and lower energy requirements for a myriad of organic transformations. Most of the small molecule photoredox catalysts fall into the category of transition metal complexes of Ru, Ir, Re, etc which have long lived triplet states. There is another class of organic photoredox catalysts gaining popularity containing lumincescent organic molecules where the properties like HOMO-LUMO band gap, (excited-state)redox potentials can be tuned very easily. However, both these family of photocatalysts lack stability during irradiation and get poisoned or photobleach after short durations. Yet another class of photocatalysts are being explored now which utilize semiconducting nanoparticles of tunable bandgap. However, functionalizing these nanoparticles for covalent attachment to other catalysts is challenging. We are developing hybrid catalysts which utilize the photophysical properties of nanoparticles and (bio)chemical properties of a protein shell like ferritin which provide them additional stability and a handle to connect with other (bio)molecules.
Assistant Professor: IISER Bhopal, India (12/2015-present)
Postdoc: University of Oxford, UK (10/2014-09/2015)
Postdoc: Leiden University, Netherlands (05/2014-09/2014)
PhD (Molecular Biophysics): Leiden University, Netherlands (10/2009-04/2014)
MA (Chemistry): Johns Hopkins University, USA (08/2006-06/2008)
MSc integrated (Chemistry): IIT Bombay, India (08/2001-06/2006)
About me: I am from Uttarakhand, a place nested in the loop of Himalayas, having spectacular scenic landscapes. I completed B.Sc.- M.Sc. from H.N.B.Garhwal University Uttarakhand in 2015. After qualifying GATE and LS-NET exam in 2016, I have joined Biocatalysis and Bioenergetics lab in December 2016 to work on my disseration leading to a PhD degree. My research interest lies in Protein engineering and Photobiocatalysis. Outside the lab, I am interested in playing games, watching movies and reading books.
About me: I am from Panipat, “the city of weavers” also known for its historical battles, land of textiles in Haryana. I completed my B.Sc. from S.D. PG College, Panipat and M.Sc. from Indian Institute of Technology, Jodhpur. After qualifying GATE in 2018, I moved to IISER Bhopal to pursue my PhD. My research interest lies in the designing of artificial enzymes by introducing novel electron-transfer pathways otherwise absent in natural systems. Besides research, I have passion for cooking, painting and playing basketball.
About me: I am from Jodhpur, heart of heritage and handloom in Rajasthan. I completed my B.Sc. from Ramjas College, University of Delhi in 2015 and M.Sc. from Panjab University, Chandigarh. After qualifying the GATE in 2017, pursuing my interest in Protein Chemistry I joined the Biocatalysis and Bioenergetics Lab at IISER Bhopal for my PhD. I am interested in studying enzyme mechanisms with a particular focus on water oxidation by rationally engineered enzymes. Besides academia, I am involved in Art and Music.
Saeed defends his MS thesis.
Welcome Akshaya and Indhu! Akshaya will carry out her iPhD project and Indhu will work on her BS/MS final year project.
We are actively looking for outstanding motivated individuals who are interesting in taking up challenges at the interface of Chemistry and Biology. If you are interested in joining our team, please send us your CV and a motivation letter or contact one of the team members for more information.
Recent graduates looking for posdoctoral positions are welcome to write to us. You must have research experience in one of the following: Bioinorganic Chemistry, Biophysical Chemistry, Protein Engineering, Reaction Mechanism, Spectroscopy, Single-Molecule spectroscopy. Generally Institute funded postdoctoral positions are not available. If you hold a fellowship or wish to apply for one, please get in touch with your CV and motivation letter and name of three referees who can comment on your academic/research history.
Students who wish to undertake BS/MS project or iPhD rotation in our lab, please talk to us well in advance as the vacancies are filled on a first-come first-served basis.
We are able to host only a limited number of visitors from IISERB and other institutions for short term. If you wish to spend time in our lab as an intern, please clearly indicate your interests/motivation when you contact us. Send us your CV and we may be able to help you.
Dr. Ankur Gupta
IISER Bhopal
Department of Chemistry
Academic Building 2, Office 19
Bhopal Bypass Road, Bhauri
Bhopal 462066
Madhya Pradesh, India